Chloride current induced by injection of calcium into Xenopus oocytes.
نویسندگان
چکیده
منابع مشابه
Dynamics of calcium regulation of chloride currents in Xenopus oocytes.
Ca-activated Cl currents are widely expressed in many cell types and play diverse and important physiological roles. The Xenopus oocyte is a good model system for studying the regulation of these currents. We previously showed that inositol 1,4,5-trisphosphate (IP3) injection into Xenopus oocytes rapidly elicits a noninactivating outward Cl current ( I Cl1-S) followed several minutes later by t...
متن کاملCalcium dependent variance of chloride current fluctuations in Xenopus laevis oocytes.
Calcium induced chloride currents were studied in Xenopus oocytes using the two-electrode voltage clamp technique. Fluctuations of chloride currents measured under voltage clamp were elicited by acetylcholine. Fluctuations were superimposed on a larger chloride current. These chloride currents are due to an increase of intracellular calcium. However, an injection of calcium into the cytoplasm e...
متن کاملAnalysis of calcium activated chloride current fluctuations in Xenopus laevis oocytes.
Fluctuations of calcium activated chloride currents were investigated in oocytes of Xenopus laevis. The method of noise analysis and the model of chloride channels activation by calcium ions were used to estimate the chloride channels lifetime and the average frequency of current fluctuations, which depends on changes of cytoplasmic calcium concentration. This current fluctuations can be evoked...
متن کاملHypotonicity activates a native chloride current in Xenopus oocytes
Xenopus oocytes are frequently utilized for in vivo expression of cellular proteins, especially ion channel proteins. A thorough understanding of the endogenous conductances and their regulation is paramount for proper characterization of expressed channel proteins. Here we detail a novel chloride current (ICl.swell) responsive to hypotonicity in Xenopus oocytes using the two-electrode voltage ...
متن کاملCalcium Current Activated by Depletion of Calcium Stores in Xenopus Oocytes
Ca(2+) currents activated by depletion of Ca(2+) stores in Xenopus oocytes were studied with a two-electrode voltage clamp. Buffering of cytosolic Ca(2+) with EGTA and MeBAPTA abolished I(Cl(Ca)) and unmasked a current in oocytes that was activated by InsP(3) or ionomycin in minutes and by thapsigargin or the chelators themselves over hours. At -60 mV in 10 mM extracellular CaCl(2), the current...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1984
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1984.sp015495